流式细胞术
信号转导
细胞生物学
磷酸化
生物
造血
表型
免疫系统
信号蛋白
细胞仪
计算生物学
免疫学
干细胞
遗传学
基因
作者
Sue Chow,David W. Hedley,T. Vincent Shankey
标识
DOI:10.1002/0471142956.cy0927s46
摘要
Signal transduction pathways link external stimuli with cellular responses, which normally regulate cell proliferation, death, and differentiation. The study of signal transduction was revolutionized through the development of phospho-specific antibodies that recognize proteins only when they are phosphorylated at specific sites. As discussed by Nolan and co-workers (UNIT ), one of the unique features of flow cytometry is its ability to perform correlated measurements of multiple phosphorylation states at the single cell level. This provides insight into the complexity of signaling networks that is not obtained by standard biochemical techniques. Furthermore, in combination with other phenotypic markers, flow cytometry can measure alterations in signaling pathways in subpopulations of cells. This clearly has wide potential for studying disorders of the hematopoietic and immune systems.
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