Objective: To investigate the problems in the usage of antimicrobial agents in a hospital and provide guidan-ce for the rational use of antimicrobial agents in clinic. Methods: Antimicrobial usage in patients who were discharged from hospital in January,2006 was analysed retrospectively by medical record reviewing. Results: 1 379 of 1 780 discharged patients (77.47%) used antimicrobial agents ,47.50% were for preventive administration;234(16.97%) patients used only1 kind of antimicrobial agent.The commonly used antimicrobial agents were the third generation cephalosporins, quinolones, penicillins, 52.57% of patients used antimicrobial agents for less than 7 days and 27.41% for more than 14 days.Samples from only 7.11% of patients used antimicrobial agents were performed antimicrobial susceptibility test. Conclusions: Some patients used antimicrobial agents irrationally ,detection rates of samples for antimicrobial susceptibillty test was low ,the usage of antimicrobial agents should be paid attention.[