Bio-Control Effect of Combining Bacillus subtilis fmbj with 2-Deoxy-D-Glucose on Rhizopus stolonifer

【Objective】 This study aims to enhance the biocontrol effect of antagonistic Bacillus subtilis fmbj on peach fruit soft rot disease caused by Rhizopus stolonifer.【Method】The synergitic effect of this antagonistic bacterium in combination with different concentrations of 2-Deoxy-D-Glucose(2-DOG) on pathogenic R.stolonifer,and the effect of 2-DOG on growth of both antagonistic bacterium and pathogen were tested in this work.【Result】 The results in vitro showed that R.stolonifer could be completely inhibited by 0.3 mg·mL-1 of 2-DOG,while B.subtilis fmbj stia grew and gathered abundantly in the wound of peach fruit at 20 mg·mL-1 of 2-DOG and presented a high 2-DOG-resistance.In vivo the results indicated that 4 mg·mL-1 of 2-DOG combined with 3.9×107CFU/mL of B.subtilis fmbj totally protected peach fruit from decay,but when only 3.9×108CFU/mL of B.subtilis fmbj or 2-DOG was used,the soft rot disease of peach fruit would occur and the disease incidence was respectively 30% and 35%.【Conclusion】Combination of B.subtilis fmbj and 2-DOG showed a more effective control on R.stolonifer than applying the B.subtilis fmbj or 2-DOG alone.This research established a significant bio-control method for peach fruit post harvest preservation.