Determination of Chlorogenic Acid and Rutin in Different Parts of Houttuynia Cordatas by HPLC

Objective:To determine the content in roots,stems and leaves of Houttuynia cordata,and provide a scientific basis for the exploitation and utilization of its resources.Method:Chlorogenic acid and rutin were analyzed on a Eclipse XDB-C18(4.6 mm×150 mm,5 μm)column.The mobile phase of chlorogenic acid was acetonitrile-0.1% phosphoric acid(12∶88),and the detection wavelength was set at 326 nm.The mobile phase of rutin was methanol-0.1% phosphoric acid(50∶50),and the detection wavelength was set at 355 nm.The flow rate was 1.0 mL · min-1.Result:The linear response ranged within 9.8-68.6 μg for chlorogenic acid(r=0.999 7),9.6-67.2 μg for rutin(r=0.998 5).Recoveries were 99.2% with RSD 1.47% for chlorogenic acid,100.1% with RSD 2.12% for rutin.Conclusion:There was a significant difference in the content of chlorogenic acid and rutin among the different parts of H.cordata.The content of chlorogenic acid and rutin in the sample from leaves was the highest than those from other parts,and it was lower in roots relatively.