纳米载体
人血清白蛋白
化学
纳米医学
纳米颗粒
药物输送
圆二色性
生物物理学
组合化学
纳米技术
有机化学
立体化学
材料科学
生物化学
生物
作者
Guangming Gong,Yan Xu,Yuanyuan Zhou,Zhengjie Meng,Guoyan Ren,Yunfeng Zhao,Xiang Zhang,Jinhui Wu,Yiqiao Hu
出处
期刊:Biomacromolecules
[American Chemical Society]
日期:2011-11-29
卷期号:13 (1): 23-28
被引量:59
摘要
A strategy to manipulate the disulfide bond breaking triggered unfolding, and subsequently assembly of human serum albumin (HSA) in a lipophilic drug-dependent manner is present. In this study, the hydrophobic region, a molecular switch of the HSA, was regulated to form HSA-paclitaxel (HSA-PTX) nanoparticles by a facile route. High-resolution transmission electron microscopy and fluorescence quenching indicate that HSA coassembled with PTX, which acts as a bridge to form core-shell nanoparticles about 50-240 nm in size, and that PTX might bind to the subdomain IIA sites of HSA. Change of ultraviolet absorption and circular dichroism spectra reveal the formation of HSA-PTX nanoparticles, which is a safety, injectable pharmaceutic nanocarrier system for tumor target. This method to prepare nanocarrier systems for hydrophobic guest molecules reveals a general principle of self-assembly for other plasma proteins and other pharmacologically active substances with poor water solubility. It also provides a basis for developing nanocarrier systems for a wide range of applications in nanomedicine, from drug delivery to bioimaging systems.
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