Effect of Different Signal Peptide on the Secretion of hTFF3 in Pichia Pastoris

To expression the native N-termius human trefoil factor 3, two kinds of Pichia pasotris expression vectors were constructed , one contained the STE13 signal sequence and another without the sequence. The two vectors were transformed into the Pichia pastoris. Molecular weight of the expressed peptides was about 13ku identified by SDS-PAGE and Western-blotting.N-terminus amino acids analysis proved one yeast expressed TFF3 with the native N-terminus and the right molecular weight. Another recombinant protein has ahybrid leader sequence GluAla at the N-termius and has two protein MS peaks.