[Objective]The experiment was designed to prove that using sugarcane clump as the immobilizing material to ferment was better than dissociative yeasts' fermentation,and at the same time to explore the fermentation effect of sugarcane and blackstrap when they were used as the fermentation culture.[Method] 12 fermentation groups were arrange to study the influence of the fermentation culture and immobilizing or not to fermentation effect.[Result]The fermentation result showed that when blackstrap was used as the fermentation culture,the average volume fraction of ethanol of the immobilized yeast in the fermentation liquor was 0.76% more than dissociative yeasts' fermentation;and when sugarcane juice was used as the fermentation culture,the average volume fraction of ethanol of the immobilized yeast in the fermentation liquor was 0.7% more than dissociative yeasts' fermentation.On the other hand,the influence of sugarcane juice and blackstrap to fermentation was almost the same.[Conclusion] Considering the cost of sugarcane juice and blackstrap integrally,blackstrap was the better choice in industrial production of ethanol.