Study on the Stability of Rehydrated-lyophilized Platelets

Objective To measure the numerical recoveries of rehydrated-lyophilized platelets.Methods The effects of time at room temperature, and external trehalose or human serum albumin concentration in drying buffer on numerical recoveries of rehydrated-lyophilized platelets were measured respectively by blood cell counter. Results The numerical recoveries of rehydrated-lyophilized platelets loaded with trehalose were stable after platelets' rehydration for 8 hours,and increased to (75.67±3.39)% with combination of 30 mM extracellular trehalose and 1% human serum albumin added in the drying buffer.Conclusion The stability of rehydrated-lyophilized platelets loaded with trehalose kept stable after rehydration of platelets for 8 hours. Trehalose in drying buffer significantly enhanced the stability of rehydrated and lyophilized platelets.