Effect of tyrphostin AG555 on the catalytic subunit activity of the recombinant human phosphoinositide 3-kinase p110β

Objective: To observe the effect of tyrphostin AG555 on the catalytic subunit activity of the recombinant human phosphoinositide 3-kinase(PI3-K) p110 β.Methods:The recombinant human PI3-K p110 β catalytic subunit was obtained by genetic engineering;the activity of PI3-K was detected by incubating the recombinant PI3-K p110 β with phosphatidylinositol-4,5-bisphosphate and [γ-()~(32)P]ATP at stable temperature;the resulting()~(32)P-radiolabeled lipids were extracted with chloroform and methanol,then assessed by the thin layer chromatography and autoradiography.Results: AG555 inhibited the catalytic subunit activity of the recombinant PI3-K p110 β in a concentration-dependent manner(2.5～20μmol/L). Conclusion: AG555 might be an potential inhibitor of PI3-K p110β which could be used as a molecular target for simply screening potential inhibitors of PI3-K.