Optimization of Extraction and Purification Technology of Cellulase from Cipangopaludina cahayensis

Cellulase was extracted from Cipangopaludina cahayensis.The extraction conditions was optimized by uniform design;and the cellulose extracted was purified by ammonium sulfate precipitation.The results showed that the optimum conditions for cellulase extraction were buffer pH 4.8;extraction time,12 min;centrifugal time,24 min.And the best saturation of ammonium sulfate for enzyme purification was 45%,as the enzymatic activity and specific activity was 651.01 U and 34.099 U / g,respectively.