[The Effect of Exosomes Secreted by Astrocytes on the Vitality of Neural Stem Cells].

OBJECTIVE: To investigate the effects of exosomes of mouse astrocytes on the viability of neural stem cells. METHODS: generation were obtained and treated with medium contained 0, 20, 40, 60 μg/mL of exosomes respectively. Screening the optimal exosome concentration for culturing neural stem cells by CCK-8 method. The optimal exosome concentration for neural stem cells was 40 μg/mL according to CCK-8 results. Then cells were intervened with 40 μg/mL of exosome in experimental group for 72 h, and the control group was added with the same volume of PBS. After intervention, the positive stem cells were labeled with EdU kit. Using the Transwell model, the number of nucleus stained by DAPI in the lower chamber in 40 μg/mL exosome treatment group and the control group were counted under a fluorescence microscope. RESULTS: <0.05). CONCLUSION: Mouse astrocyte exosomes can improve the viability of neural stem cells.