基因沉默
清脆的
生物
CRISPR干扰
结核分枝杆菌
计算生物学
RNA干扰
Cas9
基因
肺结核
遗传学
核糖核酸
医学
病理
作者
Andrew I. Wong,Jeremy M. Rock
出处
期刊:Methods in molecular biology
日期:2021-01-01
卷期号:: 343-364
被引量:22
标识
DOI:10.1007/978-1-0716-1460-0_16
摘要
The genetic basis for Mycobacterium tuberculosis pathogenesis is incompletely understood. One reason for this knowledge gap is the relative difficulty of genetic manipulation of M. tuberculosis. To close this gap, we recently developed a robust CRISPR interference (CRISPRi) platform for programmable gene silencing in mycobacteria. In this chapter, we: (1) discuss some of the advantages and disadvantages of CRISPRi relative to more traditional genetic approaches; and (2) provide a protocol for the application of CRISPRi to reduce transcription of target genes in mycobacteria.
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