细胞内
胞浆
生物
微生物学
大肠杆菌
细胞生物学
小泡
生物化学
膜
酶
基因
作者
Yi-Hsin Pang,Zhihui Cheng,Si Zhang,Shujie Li,Xueping Li,Xiaodan Li,Xiao Zhang,Xiaoxiao Li,Feng Yu,Heting Cui,Zhe Chen,Le Liu,Qing Li,Jianxiao Huang,Mingqing Zhang,Siwei Zhu,Lei Wang,Lu Feng
出处
期刊:Cell Reports
[Elsevier]
日期:2022-04-01
卷期号:39 (3): 110698-110698
被引量:13
标识
DOI:10.1016/j.celrep.2022.110698
摘要
Urinary tract infections are predominantly caused by uropathogenic Escherichia coli (UPEC). UPEC infects bladder epithelial cells (BECs) via fusiform vesicles, escapes into the cytosol to evade exocytosis, and establishes intracellular bacterial communities (IBCs) for the next round of infection. The UPEC vesicle escape mechanism remains unclear. Here we show that UPEC senses host immune responses and initiates escape by upregulating a key phospholipase. The UPEC phospholipase PldA disrupts the vesicle membrane, and pldA expression is activated by phosphate reduction in vesicles. The host phosphate transporter PIT1 is located on the fusiform vesicle membrane, transporting phosphate into the cytosol. UPEC infection upregulates PIT1 via nuclear factor κB (NF-κB), resulting in phosphate reduction. Silencing PIT1 blocks UPEC vesicle escape in BECs, inhibits IBC formation in mouse bladders, and protects mice from UPEC infection. Our results shed light on pathogenic bacteria responding to intracellular phosphate shortage and tackling host defense and provide insights for development of new therapeutic agents to treat UPEC infection.
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