伤口愈合
抗菌剂
角膜炎
巨噬细胞极化
巨噬细胞
M2巨噬细胞
抗菌活性
微生物学
化学
医学
细菌
免疫学
眼科
生物
体外
生物化学
遗传学
作者
Haixiang Huang,Yanyan Xie,Jing Zhong,Zhenyuan Fu,Peimin Wu,Xiaoqing Chen,Zhi-Qiang Xiao,Saiqun Li,Xuetao Shi,Dan Liang
标识
DOI:10.1016/j.compositesb.2024.111283
摘要
Bacterial keratitis is one of the leading causes of blindness in the world. Frequent administration of antibiotic eye drops is the first-line treatment, which has difficulties in achieving favorable outcomes in some cases due to the emerging antibiotic resistance and low bioavailability. Antimicrobial peptides have been shown to overcome antibiotic resistance through diverse bactericidal mechanisms including antibiofilm effects, enabling them to be a promising strategy to combat resistance. Here, we report a novel antibacterial strategy with collagen nanosheets (CN) loaded with the cationic antimicrobial short peptide Tet213 (Tet213-CN). In this study, we demonstrated that Tet213-CN possessed excellent properties with good adherence, high oxygen permeability, transparency, biocompatibility, and showed no ocular irritation. In vitro and in vivo assays demonstrated that Tet213-CN had enhanced antibacterial and corneal epithelial healing effects. Furthermore, we observed that Tet213-CN treatment facilitated innate immune defense by promoting M1 macrophage polarization, increasing intracellular ROS generation and proinflammatory cytokine secretion, and enhancing phagocytosis of bacteria via the TLR2/MyD88/NF-κB signaling pathway. Most impressively, Tet213-CN achieved considerable outcomes in Pseudomonas aeruginosa keratitis models with a one-time application. Overall, Tet213-CN may serve as an ideal strategy in the management of bacterial keratitis, and shed new light onto the treatment against antibiotic-resistant bacteria. In addition, these results provide a conceptual framework for a novel macrophage-based strategy in the treatment of corneal infection diseases.
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