沼渣
肥料
厌氧消化
沼气
食品科学
中层
梭状芽孢杆菌
沙门氏菌
产气荚膜梭菌
细菌
生物能源
微生物
致病菌
指示菌
梭菌
化学
粪大肠菌群
生物
农学
生物燃料
生物技术
甲烷
有机化学
遗传学
水质
生态学
作者
Anne-Marie Pourcher,Céline Druilhe,Caroline Le Maréchal,Elisabeth Repérant,Evelyne Boscher,Christine Ziebal,Laure Martin,Megane Lebreton,Sandra Rouxel,Catherine Houdayer,Sophie Le Roux,Lorine Derongs,Typhaine Poëzévara,Martine Sarrazin,Bérengère Nagard,Lorette Heurtevent,Martine Denis
标识
DOI:10.1016/j.wasman.2023.06.037
摘要
Interest in the conversion of manure in biogas via anaerobic digestion (AD) is growing, but questions remain about the biosafety of digestates. For a period of one year, we monitored the impact of three mesophilic agricultural biogas plants (BPs) mainly fed with pig manure (BP1, BP3) or bovine manure (BP2) on the physicochemical parameters, the composition of the microbial community and the concentration of bacteria (E. coli, enterococci, Salmonella, Campylobacter, Listeria monocytogenes, Clostridium perfringens, Clostridium botulinum and Clostridioides difficile). The BP2 digestate differed from those of the two other BPs with a higher nitrogen content, more total solids and greater abundance of Clostridia MBA03 and Disgonomonadacea. Persistence during digestion ranked from least to most, was: Campylobacter (1.6 to >2.9 log10 reduction, according to the BP) < E. coli (1.8 to 2.2 log10) < Salmonella (1.1 to 1.4 log10) < enterococci (0.2 to 1.2 log10) and C. perfringens (0.2 to 1 log10) < L. monocytogenes (-1.2 to 1.6 log10) < C. difficile and C. botulinum (≤0.5 log10). No statistical link was found between the reduction in the concentration of the targeted bacteria and the physicochemical and operational parameters likely to have an effect (NH3, volatile fatty acids and total solids contents, hydraulic retention time, presence of co-substrates), underlining the fact that the fate of the bacteria during mesophilic digestion depends on many interacting factors. The reduction in concentrations varied significantly over the sampling period, underlining the need for longitudinal studies to estimate the impact of AD on pathogenic microorganisms.
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