葛根素
免疫分析
色谱法
胶体金
化学
纳米技术
医学
生物
纳米颗粒
材料科学
抗体
替代医学
病理
免疫学
作者
Shi-Yan Yue,Yunxiang Liu,Rongrong Zhou,Zhen Zhan,Liping Kang,Luqi Huang,Yuan Yuan,Tiegui Nan
标识
DOI:10.1021/acs.jafc.4c00644
摘要
Radix Puerariae is a traditional Chinese medicinal material with a rich history of use in East and Southeast Asia. Puerarin, a unique component of the Pueraria genus, serves as a quality control marker for herbal medicines like Pueraria lobata and Pueraria thomsonii in China, displaying diverse pharmacological properties. This study developed puerarin colloidal gold immunoassay dipsticks utilizing an anti-puerarin monoclonal antibody, resulting in a fast and sensitive detection method with a limit of 500-1000 ng·mL-1. Evaluation using tap water-extracted P. lobata and P. thomsonii samples showed consistent results compared to LC-MS analysis. Cross-reactivity assessments of puerarin analogs revealed minimal interference, affirming the dipstick's reliability for distinguishing between the two species.
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