化学
癌胚抗原
生物相容性
介孔材料
二茂铁
电极
电化学
纳米技术
分析化学(期刊)
色谱法
材料科学
催化作用
癌症
有机化学
物理化学
内科学
医学
作者
Cheng Zheng,Jing Chen,Yizheng Zhang,Shuang Zhou,Lixing Wang,Jiao Zhou,Xiaofang Chen,Da‐Peng Yang,Yuming Hong
出处
期刊:Analytical Sciences
[Japan Society for Analytical Chemistry]
日期:2023-01-01
卷期号:39 (1): 5-11
标识
DOI:10.1007/s44211-022-00223-2
摘要
In the present work, a highly sensitive sandwich-type electrochemical immunosensor of carcinoembryonic antigen (CEA) was developed by preparing N-doped hollow mesoporous nanocarbon spheres/gold hybrids (NHMN/Au) hybridsas sensing platformand Au/ferrocene (Au/Fc) as signal amplifiers. The large surface area and high conductivity as well as good biocompatibility of NHMN/Au can increase the loading of primary antibody (Ab1) and accelerate the electron transport rate of the electrode surface, while Au can carry immobilized secondary antibodies (Ab2) and Fc derivative (Fc-SH).By using Fc-SH as response probe, the experiments show that the peak current of probe could increase after occurring the specific recognition of Ab1-CEA-Ab2, thus a novel sandwich-type immunosensor of CEA was developed. Finally, the proposed method for CEA detection was applied in human serum and the obtained results are satisfactory, indicating the developed method has important clinical applications for CEA determination.
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