谷胱甘肽
化学
细胞器
内质网
细胞内
胞浆
细胞生物学
氧化还原
分区(防火)
生物物理学
荧光
生物化学
生物
酶
物理
有机化学
量子力学
作者
Sarah Hübner,Gianluca Quargnali,Sebastian Thallmair,Pablo Rivera‐Fuentes
出处
期刊:Nature Chemistry
[Springer Nature]
日期:2023-06-15
卷期号:15 (10): 1415-1421
被引量:10
标识
DOI:10.1038/s41557-023-01249-3
摘要
Abstract Glutathione (GSH) is the main determinant of intracellular redox potential and participates in multiple cellular signalling pathways. Achieving a detailed understanding of intracellular GSH homeostasis depends on the development of tools to map GSH compartmentalization and intra-organelle fluctuations. Here we present a GSH-sensing platform for live-cell imaging, termed targetable ratiometric quantitative GSH (TRaQ-G). This chemogenetic sensor possesses a unique reactivity turn-on mechanism, ensuring that the small molecule is only sensitive to GSH in a desired location. Furthermore, TRaQ-G can be fused to a fluorescent protein to give a ratiometric response. Using TRaQ-G fused to a redox-insensitive fluorescent protein, we demonstrate that the nuclear and cytosolic GSH pools are independently regulated during cell proliferation. This sensor was used in combination with a redox-sensitive fluorescent protein to quantify redox potential and GSH concentration simultaneously in the endoplasmic reticulum. Finally, by exchanging the fluorescent protein, we created a near-infrared, targetable and quantitative GSH sensor.
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