Teleocidin Analogs Isolated from <i>Streptomyces eurocidicus</i> as Membrane-Vesicle-Regulated Natural Products

Four teleocidin analogs were isolated from Streptomyces eurocidicus, along with teleocidin B3. A combination of MS and NMR analyses elucidated their structures, revealing teleocidin A2 acetate and teleocidin B3 acetate as newly isolated metabolites. Teleocidins A2 and B3, known metabolites, exhibited weak antibacterial activities against Kocuria rhizophila and Bacillus subtilis. Notably, membrane vesicles of Burkholderia multivorans modulated the production levels of teleocidin analogs in S. eurocidicus, upregulating teleocidin biosynthesis but downregulating the subsequent acetylation step.