Differential expression of microRNAs in luteinising hormone-treated mouse TM3 Leydig cells

Testosterone is primarily produced by Leydig cells of the mammalian male gonads. The cellular functions of Leydig cells are regulated by the hypothalamus-pituitary-gonad axis, whereas the microRNA (miRNA) changes of LH-treated Leydig cells are unknown. Mouse TM3 Leydig cells were treated with LH, and deep sequencing showed that 29 miRNAs were significantly different between two groups (fold change of >1.5 or <0.5, p < .05), of which 27 were upregulated and two were downregulated. The differential expression of miR-29b-3p, miR-378b, miR-193b and miR-3695 was confirmed by quantitative real-time polymerase chain reaction. Bioinformatic analysis revealed that miRNAs regulated a large number of genes with different functions. Pathway analysis indicated that miRNAs were involved in the Wingless and INT-1, adenosine 5'-monophosphate-activated protein kinase, NF-kappa B and Toll-like receptor signalling pathways. Results showed that miRNAs might be involved in the regulation of LH to Leydig cells.