作者
Xiaoping Yang,Jesse S. Boehm,Xinping Yang,Kourosh Salehi-Ashtiani,Tong Hao,Yun Shen,Rakela Lubonja,Sapana R. Thomas,Ozan Alkan,Tashfeen Bhimdi,Thomas P. Green,Cory M. Johannessen,Serena J. Silver,Cindy Khanh Nguyen,Ryan Murray,Haley Hieronymus,Dawit Balcha,Changyu Fan,Chenwei Lin,Lila Ghamsari,Marc Vidal,William C. Hahn,David J. Hill,David E. Root
摘要
Two sequence-verified, clonal, publicly available collections of human open reading frames are reported. One collection is in a lentiviral vector for expression in mammalian cells; the other is in the Gateway vector system. Functional characterization of the human genome requires tools for systematically modulating gene expression in both loss-of-function and gain-of-function experiments. We describe the production of a sequence-confirmed, clonal collection of over 16,100 human open-reading frames (ORFs) encoded in a versatile Gateway vector system. Using this ORFeome resource, we created a genome-scale expression collection in a lentiviral vector, thereby enabling both targeted experiments and high-throughput screens in diverse cell types.