细胞生物学
运动性
酪氨酸磷酸化
细胞粘附
细胞迁移
半胱氨酸蛋白酶3
生物
酪氨酸激酶
磷脂酰肌醇
磷酸化
细胞
细胞凋亡
化学
程序性细胞死亡
信号转导
生物化学
作者
Jamie Senft,Brooke Helfer,Steven M. Frisch
出处
期刊:Cancer Research
[American Association for Cancer Research]
日期:2007-12-15
卷期号:67 (24): 11505-11509
被引量:114
标识
DOI:10.1158/0008-5472.can-07-5755
摘要
Abstract Cell migration plays an important role in tumor cell invasion and metastasis. Previously, we reported that caspase-8 contributes to cell migration and adhesion, a novel nonapoptotic function of an established apoptotic factor. Herein, we report that pro-caspase-8 is capable of restoring cell migration/adhesion to caspase-8-null cells, establishing the first biological function of a pro-caspase. The catalytic activity of caspase-8 was not required for cell motility. Stimulation of motility with epidermal growth factor induced the phosphorylation of caspase-8 on tyrosine-380 and the interaction of caspase-8 with the p85α subunit of phosphatidylinositol 3-kinase. Tyrosine-380 was required for the restoration of cell motility and cell adhesion in caspase-8-null cells, demonstrating the importance of the caspase-8–p85 interaction for these nonapoptotic functions. These results suggest that caspase-8 phosphorylation converts it from a proapoptotic factor to a cell motility factor that, through tyrosine-380, interacts with p85, an established cell migration component. [Cancer Res 2007;67(24):11505–9]
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