The Cryotop vitrification system is competent for the simultaneous cryopreservation of large numbers of pig in vitro‐produced blastocysts

Abstract This study aimed to compare the effectiveness, in terms of viability and quality post‐warming, when vitrifying in vitro‐produced (IVP) pig blastocysts with either a modified Cryotop ( n = 161; 20 blastocysts/device) or the conventional Superfine Open Pulled Straw (SOPS; n =160; 5‐6 blastocysts/device systems. Blastocyst viability, morphology, and apoptosis parameters were evaluated after 24 h of in vitro culture. The Cryotop system yields better results in terms of higher embryo viability and total cell numbers ( p < .05) and lower apoptosis ( p < .05) parameters than the SOPS procedure, defining a high effectiveness to simultaneously vitrify 20 pig IVP blastocysts at one time, thus increasing the yield of IVP blastocysts readily available for embryo transfer.