生物
八氢番茄红素合酶
八氢番茄红素脱氢酶
类胡萝卜素
光敏色素
植物烯
转录因子
基因表达
基因
生物化学
细胞生物学
生物合成
基因表达调控
发起人
抄写(语言学)
植物
红灯
语言学
哲学
作者
Yaxian Zhuge,Hongjie Sheng,Mengwei Zhang,Jinggui Fang,Suwen Lu
出处
期刊:Plant Science
[Elsevier]
日期:2023-06-01
卷期号:331: 111693-111693
被引量:4
标识
DOI:10.1016/j.plantsci.2023.111693
摘要
Phytochrome-interacting factors (PIFs) play important roles in light-mediated secondary metabolism; however, the roles of PIFs in grape fruit carotenogenesis remain unclear. Here, by identifying the PIF family genes in grapes, we focused on the role of VvPIF1 in carotenoid metabolism. During grape berry development, VvPIF1 expression was negatively correlated with carotenoid accumulation and the transcription of phytoene synthase 1/2 (VvPSY1/2), which encodes the major flux-controlling enzymes for carotenoid biosynthesis. Light significantly repressed VvPIF1 expression, but induced the expression of carotenogenic genes including VvPSY1/2. VvPIF1 functioned as a nucleus-localized protein and interacted with the light photoreceptor VvphyB. Overexpression of VvPIF1 resulted in the downregulation of the endogenous PIF1 gene, which may unexpectedly induce carotenoid accumulation and PSY expression in tobacco leaves. The transgenic grape leaves and tomato fruits with high VvPIF1 expression produced a significant decrease in carotenoid concentrations, with suppressed transcription of PSY and other carotenogenic genes. Further biochemical assays demonstrated that VvPIF1 bound directly to the promoters of VvPSY1/2 to inhibit their transcription. Collectively, we conclude that VvPIF1 negatively regulates carotenoid biosynthesis by repressing VvPSY expression in grapes. These findings shed light on the role and mode of action of PIFs in the carotenoid regulatory network of grapes.
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