益生元
拟杆菌
水解
双歧杆菌
厚壁菌
多糖
肠道菌群
发酵
拟杆菌
食品科学
化学
消化(炼金术)
生物化学
聚合度
生物
微生物学
乳酸菌
细菌
色谱法
聚合
16S核糖体RNA
遗传学
有机化学
基因
聚合物
作者
Xujiao Li,Yinan Chen,Lihua Song,Jiangmei Wang,Zibo Song,Xiao‐Fan Zhao,Changyan Zhou,Yan Wu
标识
DOI:10.1016/j.ijbiomac.2023.124175
摘要
Two hydrolyzed fractions of tamarind seed polysaccharide (TSP), denoted ETSP1 (176.68 kDa) and ETSP2 (34.34 kDa), were prepared by partial degradation via endo-xyloglucanase, and then characterized and evaluated by simulated gastrointestinal digestion in vitro. The results showed that the hydrolyzed TSPs remained indigestible in gastric and small intestinal media, and were fermented by gut microbiota, similar to the native TSP (Mw = 481.52 kDa). Although the degradation of hydrolyzed TSPs was accelerated during fermentation with a decreasing degree of polymerization, the content of produced total short-chain fatty acids (SCFAs) decreased. After fermentation, the gut microbiota composition was modified, esp. the Firmicutes/Bacteroidetes ratio decreased (1.06 vs. 0.96 vs. 0.80) with a decreasing degree of polymerization, which implied that the potential anti-obesity prebiotic effect was enhanced. At the genus level, hydrolyzed TSPs maintained similar roles as native TSP, including promoting beneficial bacteria (Bifidobacterium, Parabacteroides, and Faecalibacterium) and inhibiting enteropathogenic bacteria (Escherichia-Shigella and Dorea). Moreover, ETSP1 had additional potential due to abundant Bacteroides vulgatus (LDA = 4.68), and ETSP2 might perform better as related to Bacteroides xylanisolvens (LDA = 4.40). All these results indicated the prebiotic potential of hydrolyzed TSP with detailed information about changes in degradation and gut microbiota based on enzyme-hydrolysis.
科研通智能强力驱动
Strongly Powered by AbleSci AI