玉米赤霉烯酮
水解酶
代谢物
酶
化学
水解
真菌毒素
生物降解
泽拉诺
芥子酶
生物化学
立体化学
酶水解
生物
食品科学
有机化学
植物
动物科学
芸苔属
硫代葡萄糖苷
作者
Jin Hu,Gang Wang,Mingxuan Hou,Shilong Du,Jun Han,Yangguang Yu,Hongxia Gao,Dan He,Jimin Shi,Yin-Won Lee,Saleh A. Mohamed,Dawood H. Dawood,Qing Hong,Xin Liu,Jianhong Xu
标识
DOI:10.1021/acs.jafc.2c06410
摘要
Zearalenone (ZEN) is an estrogenic mycotoxin most frequently found in cereals that can cause reproductive disorders in livestock and pose a severe threat to animal husbandry. In this study, we isolated a ZEN-degrading Aeromicrobium strain from soil and found that ZenH, a hydrolase, is responsible for the hydrolysis of ZEN through comparative proteomics and biochemical studies. ZenH exhibited the highest similarity with lactone hydrolase ZHD607 from Phialophora americana at 21.52%. ZenH displayed maximal enzymatic activity at pH 7.0 and 55 °C with a Michaelis constant of 12.64 μM. The catalytic triad of ZenH was identified as S117-D142-H292 by molecular docking and site-directed mutagenesis. ZenH catalyzed the hydrolysis of ZEN to a novel metabolite, (S,E)-4-hydroxy-2-(10-hydroxy-6-oxoundec-1-en-1-yl)-7-oxabicyclo[4.2.0]octa-1,3,5-trien-8-one, which exhibited significantly lower estrogenic toxicity than ZEN. This study illustrates a novel ZEN-degrading enzyme and reveals a new degradation product. Furthermore, the enzyme showed good potential for detoxifying ZEN during food processing.
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