胰腺癌
免疫系统
癌症研究
肿瘤微环境
CXCR4型
腺癌
生物
肿瘤进展
医学
病理
癌症
趋化因子
免疫学
内科学
作者
Zhenyu Liao,Long‐Yun Ye,Tianjiao Li,Xing Jin,Xuan Lin,Qinglin Fei,Huiru Zhang,Saimeng Shi,Xianjun Yu,Kaizhou Jin,Weiding Wu
摘要
Abstract Macrophage is an essential part of the tumor immune microenvironment of pancreatic ductal adenocarcinoma. In our study, we explored the CXCR4 + macrophages subset on its prognosis value, immune profile and distinct function in pancreatic cancer progression. Specimens from 102 postoperative pancreatic patients were analyzed by flow cytometry or immune‐fluorescence, and the prognostic value of CXCR4 + macrophages infiltration was further determined by Cox regression. In silico analysis on TCGA, ICGC database and single‐cell sequencing of pancreatic ductal adenocarcinoma further validated our findings. We found that high CXCR4 + macrophages infiltration was associated with poor overall survival ( P < .01) and disease‐free survival ( P < .05) as an independent factor. CXCR4 + macrophages exhibited an M2 protumor phenotype with high expression of CD206. The function of CXCR4 + macrophages was further analyzed in the murine orthotopic PDAC model with its tumor promotion effect and inhibition of CD8 + T cells. Mechanistic and RNA‐seq analysis showed that CXCR4 + macrophages participated in extracellular matrix remodeling procedures and especially secreted SPARC through CXCR4/PI3K/Akt pathway promoting tumor proliferation and migration. Our study reveals that CXCR4 + macrophages infiltration is an indicator of poor prognosis of PDAC and targeting these cells was potentially crucial in immunotherapy of PDAC.
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