后代
脾脏
甲基化
表观遗传学
男科
内分泌学
DNA甲基化
细胞因子
免疫学
内科学
生物
医学
怀孕
基因表达
基因
遗传学
生物化学
作者
Jing Sun,Jia‐Bao Zhao,Chao Sun,Yuting Zhu,Ping Zhou,Shanjun Gao,Ying-Ao Fan,Hong Jiang,Qinghua Zheng,Jun Guan
出处
期刊:PubMed
日期:2022-03-01
卷期号:19 (1): 3-3
标识
DOI:10.22034/iji.2022.89946.1979
摘要
The methylation of IFN-γ and IL-4 genes is regarded as an epigenetic regulation that maintains the Th1 or Th2 phenotype.To explore the influence of prenatal administration of the staphylococcal enterotoxin B (SEB) in pregnant rats, on the IFN-γ or IL-4 expression in the offspring spleen.The SEB or PBS was administered intravenously to pregnant rats on the embryo-day 16. After normal delivery, the spleens from the fifth-day neonates and adult offspring were isolated under anesthesia. Quantitative PCR, western blot, ELISA and MeDIP-qPCR were applied to determine the levels of the splenic IFN-γ or IL-4 mRNAs, their protein levels, and methylation status, respectively.Prenatal administration of the SEB in pregnant rats decreased the levels of the splenic IFN-γ and IL-4 proteins in neonates, but increased their mRNA levels. However, prenatal administration of the SEB significantly augmented both mRNA and protein levels of the IFN-γ and IL-4 in the adult spleen. In addition, the prenatal SEB administration decreased the methylation of the splenic IFN-γ and IL-4 in adult but not neonatal offspring.The prenatal administration of SEB in pregnant rats can cause a mixed Th1 and Th2 cytokines response in the offspring spleen, and alter the cytokine expression of the Th1 and Th2 via decreasing the methylation in adult but, not neonatal offspring spleen.
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