Hyperguanyes A and B, two new PPAPs from the branches and leaves of Hypericum perforatum L. with anti-cholinesterase activities

Two new polycyclic polyprenylated acylphloroglucinols, hyperguanyes A and B (1–2) together with eight known compounds (3–10), were isolated from Hypericum perforatum L. Their structures were determined by using comprehensive spectroscopic techniques and quantum chemical calculation. The in vitro anti-cholinesterase activity of all compounds were studied. Among them, compounds 1–4, 8 and 9 exhibited anti-AchE and anti-BchE effects with IC₅₀ ranging from 0.34 ± 0.04 to 15.68 ± 0.54 μM.