Nuclear magnetic resonance (NMR) spectroscopy is the most effective means for structural analysis of substances,which is rapidly developed in recent years. This article reviews applications of one-dimensional nuclear magnetic resonance spectroscopy (1D-1H NMR),2D-1H NMR including COSY(Correlation Spectroscopy),TOCSY(Total Correlation Spectroscopy),NOESY(Nuclear Overhauser Effect Spectroscopy),and ROESY(Rotating Frame Overhauser Effect Spectroscopy) in structure analysis of cyclodextrin and cyclodextrin complexes.