Optimization of Fermentation Conditions for Glucose Oxidase Production by Aspergillus niger using Response Surface Method

To obtain the best producing glucose oxidase medium and culture conditions,three key factors influencing the glucose oxidase production: glucose,MgSO4·7H2O and speed,were determined by PlackettBurman design experiment. And then response surface methods were applied. Results showed that the optimum conditions for the fermentation by Aspergillus niger were: glucose 241g / L,NaNO33g / L,K2HPO41g / L,FeSO40. 01g / L,KCl 0. 5 g / L,MgSO4·7H2O 0. 36g / L and speed 168 r / min,under which the enzyme activity was227. 93U / g,increased 95. 9 percent.