Uniqueness and Dependence of Bacterial Communities on Microplastics: Comparison with Water, Sediment, and Soil

Revealing the dependence and uniqueness of microbial communities on microplastics could help us better understand the assembly of the microplastic microbial community in river ecosystems. In this study, we investigated the composition and ecological functions of the bacterial community on microplastics from the Three Gorges Reservoir area compared with those in water, sediment, and soil at species-level via full-length 16S rRNA gene sequencing. The results showed that the full-length 16S rRNA sequencing provided more detail and accurate taxa resolution of the bacterial community in microplastics (100%), water (99.90%), sediment (99.95%), and soil (100%). Betaproteobacteriales were the most abundant bacteria in microplastics (14.1%), water (32.3%), sediments (27.2%), and soil (21.0%). Unexpectedly, oligotrophic SAR11 clade was the third abundant bacteria (8.51%) and dominated the ecological functions of the bacterial community in water, but it was less observed on microplastics, with a relative abundance of 2.73×10-5. However, four opportunistic pathogens identified at the species level were selectively enriched on microplastics. Stenotrophomonas maltophilia was the main opportunistic pathogen on microplastics (0.29%). Sediment rather than soil and water may be contributed mostly to pathogens on microplastics. Moreover, some bacteria species with the biodegradation function of microplastics were enriched on microplastics, such as bacteria Rhodobacter sp., and endemic bacteria Luteimonas sp. The distinct bacteria composition on microplastics enhanced several ecological functions, such as xenobiotics biodegradation, which allows screening the bacteria with the biodegradation function of microplastics through long-term exposure.