胡桃醌
百里香醌
胰腺癌
细胞毒性
MTT法
细胞凋亡
细胞毒性T细胞
化学
药理学
IC50型
活力测定
毒理
医学
癌症
生物化学
体外
生物
内科学
抗氧化剂
作者
Namrata Karki,Sadhna Aggarwal,Roger A. Laine,Frank L. Greenway,Jack N. Losso
标识
DOI:10.1016/j.cbi.2020.109142
摘要
Juglone and thymoquinone are cytotoxic to pancreatic cancer cells. The aim of this study was to investigate, using an analysis of isobolograms, the type and degree of interactions between juglone and thymoquinone on MIA PaCa-2 pancreatic cancer cells. Cell viability was evaluated using the MTT assay. Cell death was determined by flow cytometry. The IC50 value for juglone and TQ in combination was found to be 24.75 μM, which was higher than juglone or TQ alone. Juglone alone killed Mia Paca-2 cells by ferroptosis. At concentrations where 10, 20 or 50% of cells were affected, there existed a moderate antagonistic relationship between juglone and TQ as indicated by the combination index (CI) value determined by the Compusyn software. At concentrations that affected 75% and 90% of cells, there were nearly an additive effect with CI value of 1.09249 and 0.92391, respectively. Moderate synergism was only seen at concentration where 95% of cells were affected, and the corresponding concentration of juglone and TQ at that combination was 40.90 μM and 511.19 μM, respectively.
科研通智能强力驱动
Strongly Powered by AbleSci AI