超重
瘤胃球菌
微生物群
乳酸菌
双歧杆菌
内科学
生物
医学
胃肠病学
生理学
肥胖
食品科学
生物信息学
发酵
作者
Sebastião Mauro Bezerra Duarte,José Tadeu Stefano,Luca Miele,Francesca Romana Ponziani,Marcela de Souza-Basqueira,L.S.R.R. Okada,Fernando Henrique da Silva Costa,Kyoko Toda,Daniel Ferraz de Campos Mazo,Éster Cerdeira Sabino,F.J. Carrilho,Antonio Gasbarrini,Cláudia Pinto Marques Souza de Oliveira
标识
DOI:10.1016/j.numecd.2017.10.014
摘要
Background and Aim The aim of the study was to compare the gut microbiomes from obese and lean patients with or without NASH to outline phenotypic differences. Methods and Results We performed a cross-sectional pilot study comprising biopsy-proven NASH patients grouped according to BMI. Microbiome DNA was extracted from stool samples, and PCR amplification was performed using primers for the V4 region of the 16S rRNA gene. The amplicons were sequenced using the Ion PGM Torrent platform, and data were analyzed using QIIME software. Macronutrient consumption was analyzed by a 7-day food record. Liver fibrosis ≥ F2 was associated with increased abundance of Lactobacilli (p = 0.0007). NASH patients showed differences in Faecalibacterium, Ruminococcus, Lactobacillus and Bifidobacterium abundance compared with the control group. Lean NASH patients had a 3-fold lower abundance of Faecalibacterium and Ruminococcus (p = 0.004), obese NASH patients were enriched in Lactobacilli (p = 0.002), and overweight NASH patients had reduced Bifidobacterium (p = 0.018). Moreover, lean NASH patients showed a deficiency in Lactobacillus compared with overweight and obese NASH patients. This group also appeared similar to the control group with regard to gut microbiome alpha diversity. Although there were qualitative differences between lean NASH and overweight/obese NASH, they were not statistically significant (p = 0.618). The study limitations included a small sample size, a food questionnaire that collected only qualitative and semi-quantitative data, and variations in group gender composition that may influence differences in FXR signaling, bile acids metabolism and the composition of gut microbiota. Conclusion Our preliminary finding of a different pathogenetic process in lean NASH patients needs to be confirmed by larger studies, including those with patient populations stratified by sex and dietary habits.
科研通智能强力驱动
Strongly Powered by AbleSci AI