污渍
阳离子聚合
化学
糖胺聚糖
染色
透明质酸
聚丙烯酰胺
聚丙烯酰胺凝胶电泳
色谱法
电泳
生物化学
生物
解剖
高分子化学
遗传学
酶
作者
João Pedro Souza Andrade,Caroline Pacheco Oliveira,Ana Tovar,Paulo Antônio de Souza Mourão,Eduardo Vilanova
标识
DOI:10.1002/elps.201700391
摘要
Abstract Cationic dyes such as toluidin blue are commonly employed to visualize glycosaminoglycans (GAGs) on electrophoresis gels; however, the carbocyanine‐based dye Stains‐all have been increasingly used to stain the non‐sulfated hyaluronic acid and other GAGs in submicrogram quantities. In this short communication, we demonstrate that Stains‐all is able to stain the most common GAGs on polyacrylamide gels with distinct and contrasting colors in a reproducible manner. We also show that this staining method is useful to identify GAGs present both in mixtures and in submicrogram quantities. Therefore, Stains‐all has shown to be useful in identifying GAGs on polyacrylamide gels with basis on their specific colors, at least on screening level.
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