STUDIES FOR PREPARATION OF COMBINED INACTIVATED VACCINE CONTAINING ROTA, CORONA VIRUSES AND TOXOID OF CLOSTRIDIUM PERFRINGENS TYPE(C)

A comprehensive study was conducted for preparation of trivalent inactivated vaccine containing bovine Rotavirus (BRV), Bovine Corona Virus (BCV), and toxoid of C. perfringens type C. Laboratory and field evaluation were achieved in laboratory animals; calves, and late pregnant cows with monitoring the active and passive antibodies in vaccinated cows and in their off springs respectively. Laboratory evaluation proved purity, safety, and high efficacy of the vaccine with no serological interference between different vaccine fractions. Field evaluation gave satisfactory results when pregnant cows vaccinated at late stage of pregnancy with high level of serum neutralizing antibodies against BRV; BCV, and C. perfringens type C. Maternal immunity passively protected the off springs during the critical period and remained protected till the end of sampling time at the 45 th day post parturition.