Bioprinting 3D functional neural tissue using human neural and induced pluripotent stem cells

Event Abstract Back to Event Bioprinting 3D functional neural tissue using human neural and induced pluripotent stem cells Qi Gu1, 2*, Eva Tomaskovic-Crook1, Rob Kapsa1, 3*, Mark Cook1, 3*, Qi Zhou2*, Gordon Wallace1 and Jeremy Crook1, 4* 1 University of Wollongong, ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, Australia 2 Institute of Zoology, Chinese Academy of Sciences, State Key Laboratory of Stem Cell and Reproductive Biology, China 3 The University of Melbourne, Department of Medicine, St Vincent’s Hospital, Australia 4 The University of Melbourne, Department of Surgery, St Vincent’s Hospital, Australia Printing stem cells with biomaterials is a promising strategy to engineer tissue for in vitro modelling and regenerative medicine[1]. Here we report a simple and reproducible approach to constructing neural tissue by printing human neural or induced pluripotent stem cells that are differentiated in situ to functional neurons and supporting neuroglia. The supporting biomaterial incorporates a novel clinically relevant gel-based bioink comprising alginate, carboxymethyl-chitosan and agarose. The printed bioink rapidly gels by stable crosslinking to form a porous 3D scaffold encapsulating stem cells for subsequent expansion and differentiation. Differentiated neurons are spontaneously active, show a bicuculline-induced increased calcium response, and are predominantly gamma-aminobutyric acid expressing. The 3D tissues will enable interrogation of neural development, function and disease, and may be adaptable for generating other neuronal and non-neuronal tissue subtypes in vitro. References:[1] Murphy, S.V. and A. Atala, 3D bioprinting of tissues and organs. Nat Biotechnol, 2014. 32(8): p. 773-785. Keywords: stem cell, Functionalization, 3D scaffold Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Biomimetic materials Citation: Gu Q, Tomaskovic-Crook E, Kapsa R, Cook M, Zhou Q, Wallace G and Crook J (2016). Bioprinting 3D functional neural tissue using human neural and induced pluripotent stem cells. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.01087 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. * Correspondence: Dr. Qi Gu, University of Wollongong, ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, New South Wales, Australia, qg863@uowmail.edu.au Dr. Rob Kapsa, University of Wollongong, ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, New South Wales, Australia, Email1 Dr. Mark Cook, University of Wollongong, ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, New South Wales, Australia, Email2 Dr. Qi Zhou, Institute of Zoology, Chinese Academy of Sciences, State Key Laboratory of Stem Cell and Reproductive Biology, Beijing, China, qzhou@ioz.ac.cn Dr. Jeremy Crook, University of Wollongong, ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, New South Wales, Australia, jcrook@uow.edu.au Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Qi Gu Eva Tomaskovic-Crook Rob Kapsa Mark Cook Qi Zhou Gordon Wallace Jeremy Crook Google Qi Gu Eva Tomaskovic-Crook Rob Kapsa Mark Cook Qi Zhou Gordon Wallace Jeremy Crook Google Scholar Qi Gu Eva Tomaskovic-Crook Rob Kapsa Mark Cook Qi Zhou Gordon Wallace Jeremy Crook PubMed Qi Gu Eva Tomaskovic-Crook Rob Kapsa Mark Cook Qi Zhou Gordon Wallace Jeremy Crook Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.