免疫组织化学
细胞凋亡
病理
H&E染色
黑色素瘤
裸鼠
染色
血管内皮生长因子
生物
医学
癌症研究
血管内皮生长因子受体
生物化学
作者
Zhengjun Cui,Ying Cen,Lifu Wang,Chao Gao
出处
期刊:Chinese journal of plastic surgery
日期:2006-03-01
卷期号:22 (2): 142-5
摘要
To investigate the effect of antisense VEGF165 infection on the growth of A375 cells in nude mice.A375 cells were injected s.c into the axilla of the nude mouse. After the tumor formed, we cut it into 16 pieces equally, then transplanted into another 15 nude mice. There were three groups: Group PBS, Group Ad-GFP, and Group Ad-aVEGF. Four weeks after interfere, the mice were sacrificed and their tumors were excised for naked eye and histological observation. The VEGF expression was checked with ISH and immunohistochemistry staining. The micro-vessel density (MVD) in tumor mass was counted by VIII factor immunohistochemistry staining.The visible and palpable nodules had developed at all the injected sites. Tumor growth speed was more slowly in Group Ad-aVEGF than that in other groups. GFP gene could express effectively in tumor mass. Ad-aVEGF infection could suppress the growth of tumors, and there were no obvious side effects. Ad-aVEGF resulted more tissue necrosis, but it had no obvious effect on cell apoptosis. VEGF expression was inhibited significantly in Group Ad-aVEGF, and MVD was decreased accordingly.Ad-aVEGF interfere may be a new method against human malignant melanoma, whose main mechanism is to induce ischemia, but not apoptosis.
科研通智能强力驱动
Strongly Powered by AbleSci AI