In order to screen cultivation plan of Pleurotus eryngii,taking Pleurotus eryngii as test material,the screening test of mother culture medium formula of pleurotus eryngii were carried out by orthogonal design.The results showed that PDA medium respectively added 30g bran,10g corn meal,5g yeast powder and 10g glucose,and that had the best culture effect for Pleurotus eryngii.