Synthesis of RGD tripeptide by the combination of chemical and enzymatic method

The method of synthesizing the active peptide RGD was adopted with the combinational strategies of chemistry and enzyme. DCC was used to produce GD dipeptide (HCl·Gly-Asp(oBzl)_2) , and then RGD tripeptide (Z-Arg-Gly-Asp(oBzl)_2) was enzymatically synthesized by trypsin. The results showed that the yield of GD dipeptide is 77.7% after 10 hours with the mole ratio of G and D is 1.5∶1, and under the low-water 1,4-butanediol solvents condition the yield of RGD is 41.9%.