Effects of E2 and P4 on prostaglandins production by glandular and stromal cells isolated from human endometrium

The glandular cells and stromal cells from late proliferative endometrium(n=7) and latesecretory endometrium(n=5) were incubated with 10-8mol/ L E2, 10-7mol/ L P4 and 10-8mol/ L E2+1O-7mol / L P4 with an aim of studying the effect of steroid hormones on prostaglandins (PGS)re-leasing from endometrium. It was demostrated that 10-8mol / L E2 and 10-8mol / L E7+1O7mol / LP4 significantly increased PG F2α:output by primary cultures of glandular cells isolated from lateproliferative endometrium. No effects was found on stromal cells from late proliferative endometrium,and stromal and glandular cells from late secretory endometrium. 10-7mol / L P4 didn’t effect thePGF2α output on glandular and stromal cells isolated from late proliferative and late secretoryendometrium PGE2 was not affected by steroid hormones.The results indicate that endometrial glandsare targets for the E2-stimulated increase in PGF2α output by late proliferative endometrium.