Euphorbia ebractolata has been used as insecticide and tuberculocide. Four crystalline compounds were isolated from the root of this plant. They were characterized by their physico-chemical properties and spectral data as: 3-acety alpha-amyrin (I), Jolkinolide B (II), ebracteolatanolide A (III) and ebracteolatanolide B (IV). I and II were isolated for the first time from E. ebracteolata Hayata. III and IV are new compounds.