The changes of surface hydrophobicity of biomaterial mixture from soy protein isolate-guar gum under the different pH,protein,salt and guar gum concentrations was studied.The results showed:when guar gum was added into one-component soy protein isolate system,surface hydrophobicity value decreased.Soluble protein surface hydrophobicity value was the highest at pH lever of 8.0 without salt.If salt was added at pH lever of 10.0,the value was higher than that under the condition of pure water,the surface hydrophobicity value increased as salt concentration increased if salt concentration was below 0.1mol/L and at pH lever of 10.0.On the contrary,the value decreased as salt concentration increased.This study laid a theoretical foundation for film-forming mechanism of biomaterial mixture from soy protein isolate-guar gum.