Overexpression of Heat Shock Protein70 Enhances Production of Antibody in Chinese Hamster Ovary Cells

The production of recombinant antibody was significantly enhanced by the method of overexpression of Heat Shock Protein 70 in Chinese Hamster Ovary(CHO) cells.The gene HSP70 was cloned from CHO/dhfr-cells,then HSP70 expression plasmid pcDNA3.1-HSP70 was constructed and transfected into CHO/dhfr-cells.CHO cell line overexpressing HSP70 stably was isolated and the expression of HSP70 gene was analyzed through RT-PCR.Anti-HBs chimeric antibody expression plasmids were transfected into the HSP70 cell line,ELISA was performed to test the production of anti-HBs antibody.It demonstrated that the overexpression of HSP70 can enhance the production of recombinant antibody in CHO/dhfr-cells(P0.05).