Analysis of Several Kinds of Cellulases by Two-dimensional Electrophoresis

The differences of proteins and protein content of commercial cellulases,and cellulases from Trichoderma reesei Rut C30 produced in this laboratory were investigated by two-dimensional electrophoresis.The results showed that the main protein of two cellulases preparations,produced in this laboratory and commercial cellulase was CBH,but they had some differences in EG.Firstly,with steam-exploded corn stover as the carbon source,the expression levels of EG Ⅰ and EG Ⅱ accounted for 18 % and 12 % of the secretome in protein content respectively.With dilute acid pretreated corn stover as the carbon source,the expression levels of EG Ⅰ and EG Ⅱ accounted for 7 % and 17 % of the secretome respectively.Then the expression levels of EGⅠ and EGⅡ,in the commercial cellulase,accounted for 6 % and 10 % of the secretome respectively.Secondly,such enzymes as XYNⅠ,XYNⅡ and XYN Ⅳwere detected in cellulase preparations from this laboratory,while commercial cellulase contained only XYN Ⅳ.The expression level of β-xylosidase(BXL)in cellulase preparations from this laboratory,was 5 times of that in commercial cellulase.