To design PCR primers with Oligo 6 and Primer Premier 5

The skill of PCR primer design with software is introduced in this paper.Based on the principle of PCR-primer design,the usage of two kinds of popular primer-design software was reviewed detailedly,including their merit,demerit and usage skills.It is recommended to use Premier Primer 5 for the usual automatic primers search,but Oligo 6 primers analysis.