Preparation of breviscapine cationic liposomes

Objective:To prepare breviscapine cationic liposomes and ordinary liposomes,optimize the formulation and investigate characteristics of the liposomes. Methods:The breviscapine liposomes were prepared by thin-film dispersion method. With the entrapment efficiencies as references,the formulation was optimized by the orthogonal design. The in vitro release profiles of the liposomes were determined. Results:The prepared cationic liposomes were stable with the mean size of (186±35) nm,the Zeta potential of (48.9±9.83) mV and the entrapment efficiencies (76.42±1.973)%. The fitting equations of the release profiles of both kinds of liposomes were lnln[1/(1-Q)]=0.779 7lnt-2.318 7(r=0.973 9) and lnln[1/(1-Q)]=0.355 3lnt-3.197(r=0.989 9),relatively. Conclusions:We have optimized formulation and obtained the stable and uniform breviscapine cationic liposomes.