The Effect of Kangxianling Prescription on the Expression of C-fos Protein of Hippocampus Apoptosis-related Factors of Pentylenetetrazol-induced Seizures Rats

Objective: To observe the spirit of traditional Chinese medicine against pentylenetetrazol-induced epileptic seizures in the hippocampus C-fos expression,and to explore traditional Chinese medicine in the treatment of epilepsy,anti-epileptic Ling as a possible mechanism.Methods: 90 wistar rats were randomly divided into blank control group(10),pentylenetetrazol(PTZ) intraperitoneally(ip) method in the model(80).The success of pentylenetetrazol-induced epileptic rats were randomly divided into five groups,and they were divided into model group,epilepsy,sodium valproate treatment group,low-dose anti-epileptic treatment group spirit,the spirit of anti-epileptic dose group,high doses of anti-epileptic Lingtreatment group.Seizures of rats,the control group,model group and treatment behavior of rats in each group,and detected by immunohistochemistry in the hippocampus of rat brain C-fos protein expression,using real-time quantitative(RealtimePCR) to detect large rat hippocampus in C-fos mRNA relative expression.Results: C-fos protein target surface density in the treatment group compared with the model group was significantly lower,and the difference was statistically significant(P0.01),but it did not reach the level of the control group(P0.01);Western medicine group and the positive dose of Chinese medicine in the target surface density compared with other groups was significantly different(P0.05);Compared between treatment groups there was no significant difference(P0.05) in the hippocampus of rats'C-fos mRNA expression than that in the model group which was significantly lower,and the difference was statistically significant(P0.05),but it did not reach the level of control group(P0.01).Conclusion: Chinese herbal compound spirit of anti-epileptic seizures disease by anti-epileptic effect may be reduced in epileptic rats brain levels of C-fos to inhibit neuronal apoptosis and to anti-epileptic effect.