Separation and Purification of Polysaccharides from Leaves of Gynura davaricata(L.) DC.

Two neutral polysaccharides(GDPs-1 and GDPs-2) and one acidic polysaccharide(GDPs-3) were separated and purified from water-soluble crude polysaccharide from the leaves of Gynura davaricata(L.) DC.by D315 resin column and subsequent Sephadex G-25 gel column chromatography.GPC analysis showed that the average molecular weights of GDPs-1,GDPs-2 and GDPs-3 were 1.06 × 104,4.17 × 103 D,and 3.74 × 103 D with a purity of 94.08%,92.3% and 99.8%,respectively.Infrared spectra preliminarily confirmed that the purified fractions were polysaccharides.GDPs-1 was characterized as α-and β-configuration,and GDPs-2 and GDPs-3 asα-configuration.Nuclear magnetic resonance(1H-NMR) results further proved that the polysaccharides contained only α-configuration.In conclusion,all these investigations can provide reference data for further studies on structural characterization and bioactivity of Gynura davaricata polysaccharides.