兰克尔
去卵巢大鼠
破骨细胞
骨吸收
内分泌学
下调和上调
内科学
化学
骨质疏松症
骨矿物
NF-κB
癌症研究
医学
雌激素
炎症
受体
生物化学
激活剂(遗传学)
基因
作者
Ah‐Ra Jang,Joon-Suk Park,Dong Kyu Kim,Jiyeon Park,Jae-Hun Ahn,Dong Yeon Kim,Tae-Sung Lee,Ji-Yoon Chang,Joo-Hee Choi,Jong‐Hwan Park
摘要
This study was conducted to investigate the inhibitory effects of the cell-free culture supernatant of Lactobacillus curvatus Wikim 38 (LC38-CS) on RANKL-induced osteoclast differentiation and bone loss in a mice model of ovariectomy-induced post-menopausal osteoporosis. LC38-CS inhibited the RANKL-induced differentiation of bone marrow-derived macrophages (BMDMs) into osteoclasts in a dose-dependent manner. F-actin ring formation and bone resorption were also reduced by LC38-CS treatment of RANKL-treated BMDMs. In addition, LC38-CS decreased the RANKL-induced activation of the TRAF6/NF-κB/MAPKs axis at the early stage and the expression of osteoclastogenesis-related genes in BMDMs treated with RANKL. PRMT1 and ADMA levels, new biomarkers for osteoclastogenesis, were decreased by LC38-CS treatment. The administration of LC38-CS increased bone volume and bone mineral density in ovariectomized mice in μ-CT analysis. These findings suggest that LC38-CS inhibited RANKL-induced osteoclast differentiation by the downregulation of molecular mechanisms and exerted anti-osteoporotic effects.
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